A pure culture is usually derived from a mixed culture by transferring a small sample into new, sterile growth medium in such a manner as to dispersethe individual cells across the medium surface or by thinning the sample many times before inoculating the new medium. The isolation of this only one kind of microorganism from mixture is called Pure Culture Techniques. Several techniques are used for the same. In order to maintain pure culture for extended periods in viable condition without any genetic change is referred as Preservation. During preservation most important factor is to stop microbial growth or at least lower the growth rate. Due to this toxic chemicals are not accumulated and hence viability of microorganism is not affected. The presentation will provide better idea on the isolation and preservation methods for pure culture.

Shivangi Ojha

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